Composite

Part:BBa_K1460008:Design

Designed by: Eric Holmes   Group: iGEM14_Cornell   (2014-08-26)


Anderson Promoter + CBP4 + pT7 + GST-CRS5 + Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 684
    Illegal BamHI site found at 279
    Illegal BamHI site found at 371
    Illegal BamHI site found at 2961
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2373


Design Notes

Expression of GST-CRS5 is controlled by the bacteriophage T7 promoter, so part should be combined with a host organism expressing T7 polymerase (such as E. coli BL21-AI from Life Technologies).


Source

This part is a composite of the parts BBa_K1460005 and BBa_K1460001.

References